WB
Gel: 8% SDS-PAGE, Lysate: 40 μg, Lane 1-5: Caco-2, MCF7, Hela, LO2 cell, Mouse brain tissue lysates, Primary antibody: DHCR7 Antibody at dilution 1/1150 Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 10 seconds.IHC
The image is immunohistochemistry of paraffin embedded Human liver cancer tissue using DHCR7 Antibody at dilution 1/80. (Original magnification: ×200)IHC
The image is immunohistochemistry of paraffin embedded Human gastric cancer tissue using DHCR7 Antibody at dilution 1/80.(Original magnification: ×200)| Product Name | Anti-DHCR7 rabbit polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of human DHCR7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DHCR7 |
|---|---|
| Gene Synonyms | SLOS |
| Gene Full Name | 7-dehydrocholesterol reductase |
| Gene Summary | This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009] |
| Alternative Names | SLOS |
| Molecular Weight(MW) | 54 kDa(Observed MW 70 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Gel: 8% SDS-PAGE, Lysate: 40 μg, Lane 1-5: Caco-2, MCF7, Hela, LO2 cell, Mouse brain tissue lysates, Primary antibody: DHCR7 Antibody at dilution 1/1150 Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 10 seconds.
IHC
The image is immunohistochemistry of paraffin embedded Human liver cancer tissue using DHCR7 Antibody at dilution 1/80. (Original magnification: ×200)
IHC
The image is immunohistochemistry of paraffin embedded Human gastric cancer tissue using DHCR7 Antibody at dilution 1/80.(Original magnification: ×200)| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C. |
| Storage Buffer | pH7.4 PBS, 0.05% NaN3, 40% Glycerol. |
