WB
Western blot analysis of Phospho-LRRK2 (S935) on different lysates with Rabbit anti-Phospho-LRRK2 (S935) antibody at 1/1,000 dilution. Lane 1: MEF cell lysate (20 µg/Lane), Lane 2: MEF treated with 1μM okadaic acid for 1 hour cell lysate (20 µg/Lane), Lane 3: MEF cell lysate (20 µg/Lane), Lane 4: MEF treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane), Lane 5: NIH/3T3 cell lysate (10 µg/Lane), Lane 6: MEF treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour (20 µg/Lane), Exposure time: 3 minutes; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of MEF cells treated with 100nM Calyculin A for 30 minutes labeling Phospho-LRRK2 (S935) with Rabbit anti-Phospho-LRRK2 (S935) antibody at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-LRRK2 (S935) antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of MEF cells (left) and MEF cells treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-LRRK2 (S935). Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Phospho-LRRK2 (S935) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser935 of human LRRK2 protein. |
| Modification | p-S935 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:1000 ICC/IF:1:1000 FC:1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LRRK2 |
|---|---|
| Gene Synonyms | PARK8 RIPK7 ROCO2 AURA17 DARDARIN |
| Gene Full Name | leucine rich repeat kinase 2 |
| Gene Summary | This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008] |
| Alternative Names | augmented in rheumatoid arthritis 17 antibody AURA17 antibody Dardarin antibody Leucine rich repeat kinase 2 antibody leucine rich repeat serine threonine protein kinase 2 antibody Leucine-rich repeat serine/threonine-protein kinase 2 antibody LRRK 2 antibody LRRK2 antibody LRRK2_HUMAN antibody PARK 8 antibody augmented in rheumatoid arthritis 17 antibody AURA17 antibody Dardarin antibody Leucine rich repeat kinase 2 antibody leucine rich repeat serine threonine protein kinase 2 antibody Leucine-rich repeat serine/threonine-protein kinase 2 antibody LRRK 2 antibody LRRK2 antibody LRRK2_HUMAN antibody PARK 8 antibody PARK8 antibody RIPK7 antibody ROCO 2 antibody ROCO2 antibody |
| Molecular Weight(MW) | 286 kDa |
| Cellular Localization | Cytoplasmic vesicle, Perikaryon, Golgi apparatus membrane, Cell projection, axon, dendrite, Endoplasmic reticulum membrane, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Endosome, Lysosome, Mitochondrion outer membrane, Cytoplasm, cytoskeleton. |
WB
Western blot analysis of Phospho-LRRK2 (S935) on different lysates with Rabbit anti-Phospho-LRRK2 (S935) antibody at 1/1,000 dilution. Lane 1: MEF cell lysate (20 µg/Lane), Lane 2: MEF treated with 1μM okadaic acid for 1 hour cell lysate (20 µg/Lane), Lane 3: MEF cell lysate (20 µg/Lane), Lane 4: MEF treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane), Lane 5: NIH/3T3 cell lysate (10 µg/Lane), Lane 6: MEF treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour (20 µg/Lane), Exposure time: 3 minutes; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of MEF cells treated with 100nM Calyculin A for 30 minutes labeling Phospho-LRRK2 (S935) with Rabbit anti-Phospho-LRRK2 (S935) antibody at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-LRRK2 (S935) antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of MEF cells (left) and MEF cells treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-LRRK2 (S935). Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 ICC/IF:1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
