WB
Western blot analysis of extracts from NIH/3T3 cells, using Spartin, SPG20 Antibody. The lane on the left was treated with blocking peptide.IHC
Spartin, SPG20 Antibody at 1/100 staining Human normal tissues adjacent to pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to Spartin, SPG20 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human Spartin, SPG20, corresponding to a region within the internal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPART |
|---|---|
| Gene Synonyms | SPG20 TAHCCP1 |
| Gene Full Name | spartin |
| Gene Summary | This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq, Nov 2008] |
| Alternative Names | SPARTIN Spastic paraplegia 20 (Troyer syndrome) TAHCCP1 Trans activated by hepatitis C virus core protein 1 |
| Molecular Weight(MW) | 75 kDa |
| Cellular Localization | Cytoplasm. Midbody. |
WB
Western blot analysis of extracts from NIH/3T3 cells, using Spartin, SPG20 Antibody. The lane on the left was treated with blocking peptide.
IHC
Spartin, SPG20 Antibody at 1/100 staining Human normal tissues adjacent to pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
