WB
Western Blot Analysis of Recombinant Firefly Luciferase Protein.WB
Direct near-infrared Western blot analysis of recombinant firefly luciferase fusion protein.| Product Name | Mouse Monoclonal antibody to Luciferase |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Firefly Luciferase Antibody is a mouse monoclonal IgG2a κ antibody against luciferase (also referred to as anti-firefly luciferase antibody). It enables the detection of luciferase protein derived from Photinus pyralis via Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF) and Enzyme-Linked Immunosorbent Assay (ELISA).
This anti-luciferase antibody is available in both unconjugated form and a variety of conjugated formats, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates.
Luciferase isolated from Photinus pyralis, a common North American firefly, is one of the most extensively studied enzymes that catalyze light production in bioluminescent organisms. As a member of the ATP-dependent AMP-binding enzyme family, it emits green light at a wavelength of 562 nm.
The chemical reaction catalyzed by luciferase is as follows: Photinus luciferin + O₂ + ATP → Oxidized Photinus luciferin + CO₂ + AMP + Pyrophosphate + Light |
| Immunogen | raised against amino acids 251-550 mapping at the C-terminus of Luciferase of Photinus pyralis origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | ELISAIFIPWB |
| WB:1:100-1:1000 ELISA:1:1000-1:10000 IP:1:10-1:100 |
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| Species Reactivity | Photinus pyralis |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Molecular Weight(MW) | 62 kDa |
|---|
WB
Western Blot Analysis of Recombinant Firefly Luciferase Protein.
WB
Direct near-infrared Western blot analysis of recombinant firefly luciferase fusion protein.| Application Notes | WB:1:100-1:1000 ELISA:1:1000-1:10000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 50 µg IgG2a kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
