WB
Mouse brain total extract lysate and 37°C incubated mouse brain membrane extract lysate were subjected to SDS PAGE followed by western blot with NKCC1/SLC12A2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using NKCC1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using NKCC1/SLC12A2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using NKCC1/SLC12A2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NKCC1/SLC12A2 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | NKCC1/SLC12A2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SLC12A2 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC12A2 |
|---|---|
| Gene Synonyms | BSC BSC2 CCC1 BSC-2 KILQS NKCC1 hNKCC1 PPP1R141 |
| Gene Full Name | solute carrier family 12 member 2 |
| Gene Summary | The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| Alternative Names | NKCC1 SLC12A2 NKCC1 SLC12A2 Basolateral Na K Cl symporter Basolateral Na-K-Cl symporter |
| Molecular Weight(MW) | 131 kDa(Observed band size: 150-160 kDa) |
| Cellular Localization | Cell membrane,Membrane |
WB
Mouse brain total extract lysate and 37°C incubated mouse brain membrane extract lysate were subjected to SDS PAGE followed by western blot with NKCC1/SLC12A2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using NKCC1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using NKCC1/SLC12A2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using NKCC1/SLC12A2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NKCC1/SLC12A2 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
