WB
Western blot analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates, Lane 2: Caco-2 whole cell lysates, Lane 3: PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-NKCC1/SLC12A2 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. NKCC1/SLC12A2 was detected in a paraffin-embedded section of rat colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-NKCC1/SLC12A2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IF-P
IF analysis using anti- SLC12A2 antibody. detected in paraffin-embedded section of human Colorectal adenocarcinoma tissues. The tissue section were stained using the Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue).| Product Name | Mouse monoclonal antibody to NKCC1/SLC12A2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human NKCC1/SLC12A2 recombinant protein (Position: K889-K943). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC12A2 |
|---|---|
| Gene Synonyms | BSC BSC2 CCC1 BSC-2 KILQS NKCC1 hNKCC1 PPP1R141 |
| Gene Full Name | solute carrier family 12 member 2 |
| Gene Summary | The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| Alternative Names | Basolateral Na K Cl symporter BSC BSC2 NKCC1 SLC12A2 |
| Molecular Weight(MW) | 131kDa(Observed MW 180-200kDa) |
| Cellular Localization | Basolateral cell membrane. |
WB
Western blot analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates, Lane 2: Caco-2 whole cell lysates, Lane 3: PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-NKCC1/SLC12A2 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody. NKCC1/SLC12A2 was detected in a paraffin-embedded section of rat colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-NKCC1/SLC12A2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IF-P
IF analysis using anti- SLC12A2 antibody. detected in paraffin-embedded section of human Colorectal adenocarcinoma tissues. The tissue section were stained using the Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
