WB
human placenta tissue were subjected to SDS PAGE followed by western blot with CD34 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using CD34 antibody at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using CD34 antibody at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD34 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using CD34 antibody at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (magenta), ASGR1 antibody labeled with FlexAble CoraLite Plus 555 Kit (orange), CoraLite® Plus 488 CD163 antibody (green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD34 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | CD34 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CD34 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | Ig2b |
| Host Species | Mouse |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:4000 IHC:1:2500-1:10000 IF-P:1:200-1:800 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD34 |
|---|---|
| Gene Full Name | CD34 molecule |
| Gene Summary | The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Alternative Names | 3C8G12 CD34 molecule |
| Molecular Weight(MW) | 41 kDa(Observed band size: 105 kDa) |
| Cellular Localization | Membrane |
WB
human placenta tissue were subjected to SDS PAGE followed by western blot with CD34 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using CD34 antibody at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using CD34 antibody at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD34 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using CD34 antibody at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (magenta), ASGR1 antibody labeled with FlexAble CoraLite Plus 555 Kit (orange), CoraLite® Plus 488 CD163 antibody (green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD34 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:1000-1:4000 IHC:1:2500-1:10000 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
