WB
Western blot analysis of various lysates, using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb (AP1343) at1:2000 dilution. HeLa and NIH/3T3 cells were treated with TGF-β3（H） (200 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.WB
Western blot analysis of various lysates using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb at 1:1000 dilution. C6 cells were treated with TGF-β3 (200 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.| Product Name | Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD2/SMAD3 |
|---|---|
| Gene Synonyms | JV18 LDS6 CHTD8 MADH2 MADR2 JV18-1 hMAD-2 hSMAD2 |
| Gene Full Name | SMAD family member 2/3 |
| Gene Summary | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012] |
| Alternative Names | HSPC193 HsT17436 JV15-2 LDS1C LDS3 MADH3 Phospho-Smad2-S465/S467 + Smad3-S423/S425 |
| Molecular Weight(MW) | 52kDa/60kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of various lysates, using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb (AP1343) at1:2000 dilution. HeLa and NIH/3T3 cells were treated with TGF-β3（H） (200 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
WB
Western blot analysis of various lysates using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb at 1:1000 dilution. C6 cells were treated with TGF-β3 (200 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
