WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Smad2/3 (Phospho Thr8) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: NIH-3T3 tearted with hTNF-α(20 ng/mL) for 30 minutes..| Product Name | Smad2/3 (Phospho Thr8) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Thr8 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SMAD2,SMAD3 |
|---|---|
| Gene Synonyms | JV18 LDS6 CHTD8 MADH2 MADR2 JV18-1 hMAD-2 hSMAD2 |
| Gene Full Name | SMAD family member 2/3 |
| Alternative Names | SMAD2 MADH2 MADR2 Mothers against decapentaplegic homolog 2 MAD homolog 2 Mothers against DPP homolog 2 JV18-1 Mad-related protein 2 hMAD-2 SMAD family member 2 SMAD 2 Smad2 hSMAD2 SMAD3 MADH3 Mothers against decapentaplegic |
| Molecular Weight(MW) | 48kD (Calculated),62kD (Observed) |
| Function | Phospho-Smad2/3 (T8) Antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at T8.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PFtPP |
| Cellular Localization | Cytoplasm . Nucleus . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Smad2/3 (Phospho Thr8) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: NIH-3T3 tearted with hTNF-α(20 ng/mL) for 30 minutes..| Application Notes | WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
