WB
Western blotting analysis using gephyrin antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with gephyrin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.FC
Flow cytometric analysis of Gephyrin expression in HAP-1 cells using Gephyrin antibody. Green, isotype control; red, Gephyrin.| Product Name | Mouse anti-GPHN Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human GPHN protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCWB |
| WB:1:500-1:2500 FC:1:200-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPHN |
|---|---|
| Gene Synonyms | GPH GEPH HKPX1 GPHRYN MOCODC |
| Gene Full Name | gephyrin |
| Gene Summary | This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq, Jul 2008] |
| Alternative Names | GPHN Gephyrin KIAA1385 GPH GEPH GPHRYN MOCODC HKPX1 |
| Cellular Localization | Cell junction, synapse, postsynaptic cell membrane; Lipid-anchor; Cytoplasmic side. Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Cell projection, dendrite. Cell junction, synapse, postsynaptic density. |
WB
Western blotting analysis using gephyrin antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with gephyrin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
FC
Flow cytometric analysis of Gephyrin expression in HAP-1 cells using Gephyrin antibody. Green, isotype control; red, Gephyrin.| Application Notes | WB:1:500-1:2500 FC:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide. |
