WB
Western blot analysis of UPF1 using anti-UPF1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UPF1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to Zebrafish UPF1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish UPF1 recombinant protein (Position: F538-D591). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | upf1 |
|---|---|
| Gene Synonyms | NORF1 rent1 zgc:55472 wu:fi40f07 wu:fj48a01 |
| Gene Full Name | UPF1 RNA helicase and ATPase |
| Gene Summary | Predicted to enable RNA binding activity and RNA helicase activity. Involved in chordate embryonic development and nuclear-transcribed mRNA catabolic process, nonsense-mediated decay. Acts upstream of or within with a positive effect on positive regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay. Acts upstream of or within T cell differentiation in thymus and regulation of gene expression. Predicted to be located in P-body; nucleus; and perinuclear region of cytoplasm. Predicted to be active in cytoplasm. Orthologous to human UPF1 (UPF1 RNA helicase and ATPase). [provided by Alliance of Genome Resources, Jul 2025] |
| Molecular Weight(MW) | 124 kDa(Observed band size: 130 kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of UPF1 using anti-UPF1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UPF1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
