WB
WB result of ATGL antibody (1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATGL transfected A431 cells.WB
Various lysates were subjected to SDS PAGE followed by western blot with ATGL antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with ATGL antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IP
IP result of anti-ATGL (IP:4ug; Detection: 1:500) with mouse testis tissue lysate 4000ug.ICC/IF
Immunofluorescent analysis of A431 cells using ATGL antibody at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.| Product Name | ATGL Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:5000-1:50000 ICC/IF:1:10-1:100 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PNPLA2 |
|---|---|
| Gene Synonyms | ATGL TTS2 PEDF-R FP17548 TTS-2.2 iPLA2zeta 1110001C14Rik |
| Gene Full Name | patatin like domain 2, triacylglycerol lipase |
| Gene Summary | This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010] |
| Alternative Names | Adipose triglyceride lipase PNPLA2 Calcium-independent phospholipase A2-zeta Desnutrin EC:3.1.1.3 |
| Molecular Weight(MW) | 55 kDa(Observed band size: 45-55 kDa) |
| Cellular Localization | Cell membrane,Cytoplasm,Lipid droplet,Membrane |
WB
WB result of ATGL antibody (1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATGL transfected A431 cells.
WB
Various lysates were subjected to SDS PAGE followed by western blot with ATGL antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with ATGL antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP
IP result of anti-ATGL (IP:4ug; Detection: 1:500) with mouse testis tissue lysate 4000ug.
ICC/IF
Immunofluorescent analysis of A431 cells using ATGL antibody at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.| Application Notes | WB:1:5000-1:50000 ICC/IF:1:10-1:100 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
