WB
Western blot analysis of lysates from Rat adipose using ATGL/PNPLA2 Rabbit mAb at 1:15000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20 s.WB
Western blot analysis of various lysates using ATGL/PNPLA2 Rabbit mAb at 1:15000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20 s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse brown adipose tissue using ATGL/PNPLA2 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat brown adipose tissue using ATGL/PNPLA2 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Confocal imaging of 3T3-L1 differentiated cells and 3T3-L1 cells using ATGL/PNPLA2 Rabbit mAb (dilution 1:400) followed by a further incubation with Cy3 conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.| Product Name | ATGL/PNPLA2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:60000 IHC:1:2000 - 1:8000 ICC/IF:1:200 - 1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PNPLA2 |
|---|---|
| Gene Synonyms | ATGL TTS2 PEDF-R FP17548 TTS-2.2 iPLA2zeta 1110001C14Rik |
| Gene Full Name | patatin like domain 2, triacylglycerol lipase |
| Gene Summary | This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010] |
| Alternative Names | ATGL TTS2 PEDF-R FP17548 TTS-2.2 iPLA2zeta 1110001C14Rik ATGL/PNPLA2 |
| Molecular Weight(MW) | 20 kDa/55 kDa(Observed band size:54 kDa) |
| Cellular Localization | Cell membrane, Lipid droplet, Single-pass membrane protein, Cytoplasm. |
WB
Western blot analysis of lysates from Rat adipose using ATGL/PNPLA2 Rabbit mAb at 1:15000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20 s.
WB
Western blot analysis of various lysates using ATGL/PNPLA2 Rabbit mAb at 1:15000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20 s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse brown adipose tissue using ATGL/PNPLA2 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat brown adipose tissue using ATGL/PNPLA2 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Confocal imaging of 3T3-L1 differentiated cells and 3T3-L1 cells using ATGL/PNPLA2 Rabbit mAb (dilution 1:400) followed by a further incubation with Cy3 conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.| Application Notes | WB:1:5000-1:60000 IHC:1:2000 - 1:8000 ICC/IF:1:200 - 1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
