IHC
Phospho-CFTR (Thr1471) Antibody at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.ICC/IF
Phospho-CFTR (Thr1471) Antibody staining H2O2 treated HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Phospho-CFTR (Thr1471) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human CFTR around the phosphorylation site of Thr1471. |
| Modification | p-Thr1471 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CFTR |
|---|---|
| Gene Synonyms | CF MRP7 ABC35 ABCC7 CFTR/MRP TNR-CFTR dJ760C5.1 |
| Gene Full Name | CF transmembrane conductance regulator |
| Gene Summary | This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017] |
| Alternative Names | ABC 35 ABC35 ABCC 7 ABCC7 ATP binding cassette sub family C member 7 ATP Binding Cassette Superfamily C Member 7 ATP binding cassette transporter sub family C member 7 ATP-binding cassette sub-family C member 7 cAMP dependent chloride channel cAMP-dependent chloride channel CF CFTR CFTR/MRP CFTR_HUMAN Channel conductance controlling ATPase Channel conductance-controlling ATPase Cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub family C member 7) Cystic fibrosis transmembrane conductance regulator Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 Cystic Fibrosis Transmembrane Regulator dJ760C5.1 MRP 7 MRP7 TNR CFTR |
| Molecular Weight(MW) | 168 kDa |
| Cellular Localization | Apical cell membrane>Multi-pass membrane protein. Early endosome membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Recycling endosome membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Nucleus. |
IHC
Phospho-CFTR (Thr1471) Antibody at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
ICC/IF
Phospho-CFTR (Thr1471) Antibody staining H2O2 treated HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
