WB
Western blot analysis of CFTR on different lysates with Rabbit anti-CFTR antibody at 1/2,000 dilution. Lane 1: Caco-2 cell lysate, Lane 2: HT-29 cell lysate, Lane 3: U-2 OS cell lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-CFTR antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Species: Human, Site: pancreas, Sample: Paraffin-embedded section, Antibody concentration: 1/500.| Product Name | CFTR Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CFTR aa 1,181-1,440. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:2000 IHC:1:200 IF-P:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CFTR |
|---|---|
| Gene Synonyms | CF MRP7 ABC35 ABCC7 CFTR/MRP TNR-CFTR dJ760C5.1 |
| Gene Full Name | CF transmembrane conductance regulator |
| Gene Summary | This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017] |
| Alternative Names | ABC 35 antibody ABC35 antibody ABCC 7 antibody ABCC7 antibody ATP binding cassette sub family C member 7 antibody ATP Binding Cassette Superfamily C Member 7 antibody ATP binding cassette transporter sub family C member 7 antibody ATP-binding cassette sub-family C member 7 antibody cAMP dependent chloride channel antibody cAMP-dependent chloride channel antibody ABC 35 antibody ABC35 antibody ABCC 7 antibody ABCC7 antibody ATP binding cassette sub family C member 7 antibody ATP Binding Cassette Superfamily C Member 7 antibody ATP binding cassette transporter sub family C member 7 antibody ATP-binding cassette sub-family C member 7 antibody cAMP dependent chloride channel antibody cAMP-dependent chloride channel antibody CF antibody CFTR antibody CFTR/MRP antibody CFTR_HUMAN antibody Channel conductance controlling ATPase antibody Channel conductance-controlling ATPase antibody Cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub family C member 7) antibody Cystic fibrosis transmembrane conductance regulator antibody Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 antibody Cystic Fibrosis Transmembrane Regulator antibody dJ760C5.1 antibody MRP 7 antibody MRP7 antibody TNR CFTR antibody |
| Molecular Weight(MW) | 168 kDa(Observed band size: 250 kDa) |
| Cellular Localization | Apical cell membrane, Early endosome membrane, Cell membrane, Recycling endosome membrane, Endoplasmic reticulum membrane, Nucleus. |
WB
Western blot analysis of CFTR on different lysates with Rabbit anti-CFTR antibody at 1/2,000 dilution. Lane 1: Caco-2 cell lysate, Lane 2: HT-29 cell lysate, Lane 3: U-2 OS cell lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-CFTR antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Species: Human, Site: pancreas, Sample: Paraffin-embedded section, Antibody concentration: 1/500.| Application Notes | WB:1:2000 IHC:1:200 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
