WB
Western blot analysis of GIRK2 on different lysates with Rabbit anti-GIRK2 antibody at 1/1,000 dilution.Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane.Exposure time: 15 seconds;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IF-F
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.IP
GIRK2 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with GIRK2 Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using GIRK2 Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input); Lane 2: GIRK2 Recombinant Rabbit Monoclonal Antibody IP in mouse brain tissue lysate; Lane 3: Rabbit IgG instead of GIRK2 Recombinant Rabbit Monoclonal Antibody in mouse brain tissue lysate. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 26 seconds;IHC
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GIRK2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GIRK2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GIRK2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | GIRK2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCIPWB |
| WB:1:1000 IHC:1:1000 IF-F:1:500 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KCNJ6 |
|---|---|
| Gene Synonyms | BIR1 GIRK2 KATP2 KCNJ7 KPLBS GIRK-2 KATP-2 KIR3.2 hiGIRK2 |
| Gene Full Name | potassium inwardly rectifying channel subfamily J member 6 |
| Gene Summary | This gene encodes a member of the G protein-coupled inwardly-rectifying potassium channel family of inward rectifier potassium channels. This type of potassium channel allows a greater flow of potassium into the cell than out of it. These proteins modulate many physiological processes, including heart rate in cardiac cells and circuit activity in neuronal cells, through G-protein coupled receptor stimulation. Mutations in this gene are associated with Keppen-Lubinsky Syndrome, a rare condition characterized by severe developmental delay, facial dysmorphism, and intellectual disability. [provided by RefSeq, Apr 2015] |
| Alternative Names | inwardly rectifying subfamily J member 6 antibody BIR1 antibody G protein activated inward rectifier potassium channel 2 antibody G protein-activated inward rectifier potassium channel 2 antibody GIRK-2 antibody GIRK2 antibody hiGIRK2 antibody Inward rectifier K(+) channel Kir3.2 antibody Inward rectifier potassium channel KIR3.2 antibody KATP-2 antibody inwardly rectifying subfamily J member 6 antibody BIR1 antibody G protein activated inward rectifier potassium channel 2 antibody G protein-activated inward rectifier potassium channel 2 antibody GIRK-2 antibody GIRK2 antibody hiGIRK2 antibody Inward rectifier K(+) channel Kir3.2 antibody Inward rectifier potassium channel KIR3.2 antibody KATP-2 antibody KATP2 antibody KCNJ6 antibody KCNJ6_HUMAN antibody Kcnj7 antibody KIR3.2 antibody KPLBS antibody Potassium channel antibody Potassium channel inwardly rectifying subfamily J member 6 antibody Potassium inwardly rectifying channel subfamily J member 6 antibody Potassium voltage gated channel subfamily J member 6 antibody |
| Molecular Weight(MW) | 48 kDa(Observed band size: 35/48 kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of GIRK2 on different lysates with Rabbit anti-GIRK2 antibody at 1/1,000 dilution.Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane.Exposure time: 15 seconds;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IF-F
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
IP
GIRK2 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with GIRK2 Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using GIRK2 Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input); Lane 2: GIRK2 Recombinant Rabbit Monoclonal Antibody IP in mouse brain tissue lysate; Lane 3: Rabbit IgG instead of GIRK2 Recombinant Rabbit Monoclonal Antibody in mouse brain tissue lysate. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 26 seconds;
IHC
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GIRK2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GIRK2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GIRK2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:1000 IF-F:1:500 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
