WB
Western blot analysis of Phospho-Chk2 (T68) on different lysates with Rabbit anti-Phospho-Chk2 (T68) antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 20μM Etoposide for 2 hours cell lysate, Lane 3: Jurkat cell lysate, Lane 4: Jurkat treated with 25μM Etoposide for 5 hours cell lysate, Lane 5: NIH/3T3 cell lysate, Lane 6: NIH/3T3 treated with 25μM Etoposide for 5 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Phospho-Chk2 (T68) on different lysates with Rabbit anti-Phospho-Chk2 (T68) antibody at 1/1,000 dilution. Lane 1: C6 cell lysate, Lane 2: C6 treated with 25μM Etoposide for 5 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-Chk2 (T68) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Thr68 of human Chk2. |
| Modification | p-T68 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CHEK2 |
|---|---|
| Gene Synonyms | CDS1 CHK2 LFS2 RAD53 TPDS4 hCds1 HuCds1 PP1425 |
| Gene Full Name | checkpoint kinase 2 |
| Gene Summary | In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| Alternative Names | CDS 1 antibody Cds1 antibody Cds1 homolog antibody Checkpoint kinase 2 antibody Checkpoint like protein CHK2 antibody CHEK 2 antibody Chek2 antibody Chk 2 antibody CHK2 checkpoint homolog (S. pombe) antibody CHK2 checkpoint homolog antibody CDS 1 antibody Cds1 antibody Cds1 homolog antibody Checkpoint kinase 2 antibody Checkpoint like protein CHK2 antibody CHEK 2 antibody Chek2 antibody Chk 2 antibody CHK2 checkpoint homolog (S. pombe) antibody CHK2 checkpoint homolog antibody CHK2_HUMAN antibody hCds1 antibody HuCds 1 antibody LFS 2 antibody LFS2 antibody PP1425 antibody RAD 53 antibody RAD53 antibody Rad53 homolog antibody Serine/threonine protein kinase Chk2 antibody Serine/threonine-protein kinase Chk2 antibody |
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of Phospho-Chk2 (T68) on different lysates with Rabbit anti-Phospho-Chk2 (T68) antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 20μM Etoposide for 2 hours cell lysate, Lane 3: Jurkat cell lysate, Lane 4: Jurkat treated with 25μM Etoposide for 5 hours cell lysate, Lane 5: NIH/3T3 cell lysate, Lane 6: NIH/3T3 treated with 25μM Etoposide for 5 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Phospho-Chk2 (T68) on different lysates with Rabbit anti-Phospho-Chk2 (T68) antibody at 1/1,000 dilution. Lane 1: C6 cell lysate, Lane 2: C6 treated with 25μM Etoposide for 5 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
