WB
Western blot analysis of SOS1 using anti-SOS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody.IHC
IHC analysis of SOS1 using anti-SOS1 antibody. SOS1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-SOS1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SOS1 using anti-SOS1 antibody. SOS1 was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-SOS1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SOS1 using anti-SOS1 antibody. SOS1 was detected in a paraffin-embedded section of rat cerebellum tissue. The tissue section was incubated with rabbit anti-SOS1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of K562 cells using anti-SOS1 antibody. Overlay histogram showing K562 cells stained with anti-SOS1 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SOS1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to SOS1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SOS1 recombinant protein (Position: H177-A1288). Human SOS1 shares 96.5% amino acid (aa) sequence identity with mouse SOS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOS1 |
|---|---|
| Gene Synonyms | GF1 HGF NS4 GGF1 GINGF SOS-1 |
| Gene Full Name | SOS Ras/Rac guanine nucleotide exchange factor 1 |
| Gene Summary | This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4. [provided by RefSeq, Jul 2008] |
| Alternative Names | SOS1 Son of sevenless homolog 1 SOS-1 |
| Molecular Weight(MW) | 152 kDa(Observed band size: 170 kDa) |
WB
Western blot analysis of SOS1 using anti-SOS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody.
IHC
IHC analysis of SOS1 using anti-SOS1 antibody. SOS1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-SOS1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SOS1 using anti-SOS1 antibody. SOS1 was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-SOS1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SOS1 using anti-SOS1 antibody. SOS1 was detected in a paraffin-embedded section of rat cerebellum tissue. The tissue section was incubated with rabbit anti-SOS1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of K562 cells using anti-SOS1 antibody. Overlay histogram showing K562 cells stained with anti-SOS1 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SOS1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
