WB
Western blot analysis of SOS1 on different lysates with Rabbit anti-SOS1 antibody at 1/1,000 dilution.Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SOS1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | SOS1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human SOS1 aa 1-150/1,333. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SOS1 |
|---|---|
| Gene Synonyms | GF1 HGF NS4 GGF1 GINGF SOS-1 |
| Gene Full Name | SOS Ras/Rac guanine nucleotide exchange factor 1 |
| Gene Summary | This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4. [provided by RefSeq, Jul 2008] |
| Alternative Names | alternate SOS1 antibody GF1 antibody GGF1 antibody GINGF antibody gingival fibromatosis antibody gingival fibromatosis hereditary 1 antibody Guanine nucleotide exchange factor antibody HGF antibody NS4 antibody Son of sevenless homolog 1 (Drosophila) antibody alternate SOS1 antibody GF1 antibody GGF1 antibody GINGF antibody gingival fibromatosis antibody gingival fibromatosis hereditary 1 antibody Guanine nucleotide exchange factor antibody HGF antibody NS4 antibody Son of sevenless homolog 1 (Drosophila) antibody Son of sevenless homolog 1 antibody SOS Ras/Rac guanine nucleotide exchange factor 1 antibody SOS-1 antibody Sos1 antibody SOS1_HUMAN antibody |
| Molecular Weight(MW) | 152 kDa |
| Cellular Localization | Cytosol, plasma membrane, cytoplasm, neuronal cell body, postsynaptic density. |
WB
Western blot analysis of SOS1 on different lysates with Rabbit anti-SOS1 antibody at 1/1,000 dilution.Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SOS1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
