WB
PMCA1/4: Western blot analysis of PMCA1/4 expression in JAR (A) and Hep G2 (B) whole cell lysates.IHC
PMCA1/4 Antibody: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human fallopian tube tissue shows glandular cell membrane and cytoplasmic staining.IHC
PMCA1/4 Antibody: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue demonstrates membranous and cytoplasmic staining.IHC
PMCA1/4 Antibody: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testicular tissue exhibits membranous and cytoplasmic staining in seminiferous tubule cells, as well as cytoplasmic staining in Leydig cells.| Product Name | Mouse Monoclonal antibody to PMCA1/4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | amino acids 719-738 of PMCA4b of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP2B4 |
|---|---|
| Gene Synonyms | MXRA1 PMCA4 ATP2B2 PMCA4b PMCA4x |
| Gene Full Name | ATPase plasma membrane Ca2+ transporting 4 |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 129-140 kDa |
| Cellular Localization | Cell membrane,Cell projection,Cilium,Flagellum,Membrane |
WB
PMCA1/4: Western blot analysis of PMCA1/4 expression in JAR (A) and Hep G2 (B) whole cell lysates.
IHC
PMCA1/4 Antibody: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human fallopian tube tissue shows glandular cell membrane and cytoplasmic staining.
IHC
PMCA1/4 Antibody: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue demonstrates membranous and cytoplasmic staining.
IHC
PMCA1/4 Antibody: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testicular tissue exhibits membranous and cytoplasmic staining in seminiferous tubule cells, as well as cytoplasmic staining in Leydig cells.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
