WB
THP-1 cells were subjected to SDS PAGE followed by western blot with RIPK3 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RIPK3 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using RIPK3 antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) .| Product Name | RIPK3 Recombinant monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human RIPK3 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:6000 IHC:1:200-1:800 ICC/IF:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RIPK3 |
|---|---|
| Gene Synonyms | RIP3 |
| Gene Full Name | receptor interacting serine/threonine kinase 3 |
| Gene Summary | The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008] |
| Alternative Names | RIP3 EC:2.7.11.1 Receptor interacting protein 3 Receptor-interacting protein 3 Receptor-interacting serine/threonine-protein kinase 3 |
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
THP-1 cells were subjected to SDS PAGE followed by western blot with RIPK3 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RIPK3 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using RIPK3 antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) .| Application Notes | WB:1:1000-1:6000 IHC:1:200-1:800 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
