IHC
Immunohistochemistry analysis of paraffin embedded Human colon using Phospho-RIP3 S227 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.ICC/IF
Immunofluorescence analysis of HT-29 cells (treated with Z-VAD, hTNF-α and SM-164) and HT-29 cells (untreated) using Phospho RIP3-S227 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Phospho-RIP3-S227 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | p-S227 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| IHC:1:50 - 1:200 ICC/IF:1:50 - 1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RIPK3 |
|---|---|
| Gene Synonyms | RIP3 |
| Gene Full Name | receptor interacting serine/threonine kinase 3 |
| Gene Summary | The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008] |
| Alternative Names | RIP3 Phospho-RIP3-S227 |
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Cell membrane, Cytoplasm, Mitochondrion, cytosol. |
IHC
Immunohistochemistry analysis of paraffin embedded Human colon using Phospho-RIP3 S227 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of HT-29 cells (treated with Z-VAD, hTNF-α and SM-164) and HT-29 cells (untreated) using Phospho RIP3-S227 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | IHC:1:50 - 1:200 ICC/IF:1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
