WB
Western blot analysis of NRCAM using anti-NRCAM antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NRCAM antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of Neuro2a cells using anti-NRCAM antibody. Overlay histogram showing Neuro2a cells stained with anti-NRCAM antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NRCAM Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to NRCAM |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human NRCAM recombinant protein (Position: R207-Q1025). Mouse NRCAM shares 91.7% and 97.1% amino acid (aa) sequence identity with human and rat NRCAM, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:500-1:2000 Fixed:1:50-1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Nrcam |
|---|---|
| Gene Synonyms | Bravo mKIAA0343 C030017F07Rik C130076O07Rik |
| Gene Full Name | neuronal cell adhesion molecule |
| Gene Summary | Enables protein binding activity involved in heterotypic cell-cell adhesion. Involved in cell-cell adhesion; nervous system development; and regulation of postsynapse organization. Acts upstream of or within several processes, including neuron development; neuronal action potential propagation; and regulation of neuron projection development. Located in axon; plasma membrane; and synapse. Is active in glutamatergic synapse and postsynaptic density membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and sensory organ. Used to study autism spectrum disorder. Orthologous to human NRCAM (neuronal cell adhesion molecule). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Ng-CAM-related Ng CAM related Neuronal surface protein Bravo neuronal cell adhesion molecule KIAA0343 |
| Molecular Weight(MW) | 144 kDa(Observed band size: 136 kDa) |
| Cellular Localization | Cell membrane,Cell projection,Membrane,Secreted |
WB
Western blot analysis of NRCAM using anti-NRCAM antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NRCAM antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of Neuro2a cells using anti-NRCAM antibody. Overlay histogram showing Neuro2a cells stained with anti-NRCAM antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NRCAM Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 Fixed:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
