WB
Western blot analysis of Transmembrane protein 200A on different lysates with Mouse anti-Transmembrane protein 200A antibody at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.