IHC
Paraformaldehyde-fixed, paraffin embedded (Mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPT) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Product Name | CENPT Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human CENPT : 63-72/561. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:100-1:500 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CENPT |
|---|---|
| Gene Synonyms | SSMGA CENP-T C16orf56 |
| Gene Full Name | centromere protein T |
| Gene Summary | The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPT is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008] |
| Alternative Names | C16orf56 CENP-T SSMGA CENPT_HUMAN CENPT Interphase centromere complex protein 22 ICEN22 centromere protein T chromosome 16 open reading frame 56 |
| Molecular Weight(MW) | 62kDa |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Note=Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore, and may connect it to the outer kinetochore via its N-terminus. |
IHC
Paraformaldehyde-fixed, paraffin embedded (Mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CENPT) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Application Notes | IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
