WB
WB analysis using CENPT Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CENPT Polyclonal antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IP
IP experiment of HEK-293T using CENPT Polyclonal antibody. IP result of anti-CENPT with HEK-293T cells lysate 1840 ug.| Product Name | CENPT Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CENPT protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:8000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CENPT |
|---|---|
| Gene Synonyms | SSMGA CENP-T C16orf56 |
| Gene Full Name | centromere protein T |
| Gene Summary | The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPT is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008] |
| Alternative Names | C16orf56 CENP-T Centromere protein T ICEN22 Interphase centromere complex protein 22 |
| Molecular Weight(MW) | 561 aa,60 kDa(Observed band size:70 kDa) |
| Cellular Localization | Centromere,Chromosome,Kinetochore,Nucleus. |
WB
WB analysis using CENPT Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CENPT Polyclonal antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP
IP experiment of HEK-293T using CENPT Polyclonal antibody. IP result of anti-CENPT with HEK-293T cells lysate 1840 ug.| Application Notes | WB:1:1000-1:8000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
