WB
HeLa cells were subjected to SDS PAGE followed by western blot with NFKBIZ Polyclonal antibody (NFKBIZ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with NFKBIZ Polyclonal antibody(NFKBIZ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFKBIZ antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).| Product Name | NFKBIZ Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human NFKBIZ protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIZ |
|---|---|
| Gene Synonyms | IKBZ INAP ikB-zeta ikappaBzeta IkappaB-zeta I-kappa-B-zeta |
| Gene Full Name | NFKB inhibitor zeta |
| Gene Summary | This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | I kappa B zeta IkappaB zeta IkappaBzeta I-kappa-B-zeta IkB zeta |
| Molecular Weight(MW) | 78 kDa(Observed band size:78 kDa, 64 kDa ) |
| Cellular Localization | Nucleus. |
WB
HeLa cells were subjected to SDS PAGE followed by western blot with NFKBIZ Polyclonal antibody (NFKBIZ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with NFKBIZ Polyclonal antibody(NFKBIZ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFKBIZ antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).| Application Notes | WB:1:500-1:1000 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
