WB
Western blot analysis of anti-NFKBIZ antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFKBIZ antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of NFKBIZ using anti-NFKBIZ antibody and anti-Beta Tubulin antibody . NFKBIZ was detected in an immunocytochemical section of SiHa cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green) were used as secondary antibody.FC
Flow Cytometry analysis of HEL cells using anti-NFKBIZ antibody. Overlay histogram showing HEL cells stained with Rabbit polyclonal antibody to NFKBIZ (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFKBIZ Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG(1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to NFKBIZ |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human NFKBIZ recombinant protein (Position: Q247-H577). Human NFKBIZ shares 73.2% amino acid (aa) sequence identity with mouse NFKBIZ. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIZ |
|---|---|
| Gene Synonyms | IKBZ INAP ikB-zeta ikappaBzeta IkappaB-zeta I-kappa-B-zeta |
| Gene Full Name | NFKB inhibitor zeta |
| Gene Summary | This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | NFKBIZ IKBZ INAP NF-kappa-B inhibitor zeta I-kappa-B-zeta IkB-zeta IkappaBzeta IL-1 inducible nuclear ankyrin-repeat protein INAP Molecule possessing ankyrin repeats induced by lipopolysaccharide |
| Molecular Weight(MW) | 78 kDa(Observed band size:85 kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of anti-NFKBIZ antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFKBIZ antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of NFKBIZ using anti-NFKBIZ antibody and anti-Beta Tubulin antibody . NFKBIZ was detected in an immunocytochemical section of SiHa cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green) were used as secondary antibody.
FC
Flow Cytometry analysis of HEL cells using anti-NFKBIZ antibody. Overlay histogram showing HEL cells stained with Rabbit polyclonal antibody to NFKBIZ (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFKBIZ Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG(1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
