WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 μg per lane) were separated by SDS-PAGE and immunoblotted with anti-ID2.IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ID2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.ICC/IF
Anti-ID2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ID2.| Product Name | ID2 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human ID2 (NP_002157) produced in E.coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500 IHC:1:50 ICC/IF:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | ID2 |
|---|---|
| Gene Synonyms | GIG8 ID2A ID2H bHLHb26 |
| Gene Full Name | inhibitor of DNA binding 2 |
| Gene Summary | The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq, Aug 2011] |
| Alternative Names | bHLHb26 GIG8 ID2A ID2H |
| Molecular Weight(MW) | 14.7 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 μg per lane) were separated by SDS-PAGE and immunoblotted with anti-ID2.
IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ID2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
ICC/IF
Anti-ID2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ID2.| Application Notes | WB:1:500 IHC:1:50 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
