WB
Western blot analysis of ID2 on different lysates with Mouse anti-ID2 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin- embedded human pancreatic cancer tissue using anti-ID2 Mouse mAb.| Product Name | ID2 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein with Human ID2 aa 1-80 / 134. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:1000-1:10000 IHC:1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ID2 |
|---|---|
| Gene Synonyms | GIG8 ID2A ID2H bHLHb26 |
| Gene Full Name | inhibitor of DNA binding 2 |
| Gene Summary | The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq, Aug 2011] |
| Alternative Names | bHLHb26 antibody Cell growth inhibiting gene 8 antibody class B basic helix loop helix protein 26 antibody Class B basic helix-loop-helix protein 26 antibody DNA binding protein inhibitor ID 2 antibody DNA binding protein inhibitor ID2 antibody DNA-binding protein inhibitor ID-2 antibody GIG 8 antibody GIG8 antibody Helix loop helix protein ID2 antibody ID2 antibody ID2_HUMAN antibody ID2A antibody ID2H antibody Inhibitor of differentiation 2 antibody Inhibitor of DNA binding 2 antibody Inhibitor of DNA binding 2 dominant negative helix loop helix protein antibody MGC26389 antibody |
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Cytoplasm, nucleus. |
| SwissProt ID | Q02363 |
|---|
WB
Western blot analysis of ID2 on different lysates with Mouse anti-ID2 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin- embedded human pancreatic cancer tissue using anti-ID2 Mouse mAb.| Application Notes | WB:1:1000-1:10000 IHC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
