WB
Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody at 1/2,000 dilution. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Knockdown (KD) Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody at 1/2,000 dilution. Exposure time: 78 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was incubated at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | cIAP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human cIAP1 aa 201-618. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BIRC2 |
|---|---|
| Gene Synonyms | API1 MIHB HIAP2 IAP-2 RNF48 cIAP1 Hiap-2 c-IAP1 |
| Gene Full Name | baculoviral IAP repeat containing 2 |
| Gene Summary | The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| Alternative Names | API 1 antibody API1 antibody Apoptosis inhibitor 1 antibody Baculoviral IAP repeat containing 2 antibody Baculoviral IAP repeat containing protein 2 antibody Baculoviral IAP repeat-containing protein 2 antibody BIRC 2 antibody BIRC2 antibody BIRC2_HUMAN antibody C IAP1 antibody API 1 antibody API1 antibody Apoptosis inhibitor 1 antibody Baculoviral IAP repeat containing 2 antibody Baculoviral IAP repeat containing protein 2 antibody Baculoviral IAP repeat-containing protein 2 antibody BIRC 2 antibody BIRC2 antibody BIRC2_HUMAN antibody C IAP1 antibody C-IAP1 antibody Cellular inhibitor of apoptosis 1 antibody cellular inhibitor of apoptosis protein 1 antibody cIAP 1 antibody cIAP1 antibody HIAP 2 antibody HIAP-2 antibody HIAP2 antibody IAP 2 antibody IAP homolog B antibody IAP-2 antibody IAP2 antibody Inhibitor of apoptosis protein 2 antibody MIHB antibody NFR2 TRAF signalling complex protein antibody RING finger protein 48 antibody RNF 48 antibody RNF48 antibody TNFR2 TRAF signaling complex protein 2 antibody TNFR2-TRAF-signaling complex protein 2 antibody |
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody at 1/2,000 dilution. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Knockdown (KD) Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody at 1/2,000 dilution. Exposure time: 78 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was incubated at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
