Application
Immunohistochemistry of cIAP in human lung cells with cIAP antibody at 10 μg/mL.Application
Immunofluorescence of cIAP in Human Lung cells with cIAP antibody at 20 μg/mL.Application
Western blot analysis of c-IAP in human lung lysate with c-IAP antibody at 1 (lane A), 2 (lane B), and 4 (lane C) μg/mL, respectively.| Product Name | cIAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cIAP Antibody: API1, MIHB, HIAP2, RNF48, cIAP1, Hiap-2, c-IAP1, API1, IAP2, Baculoviral IAP repeat-containing protein 2, C-IAP1, IAP-2 |
| Product description | cIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-α by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.1) Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90. |
| Immunogen | c-IAP antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human c-IAP1 c-IAP antibody detects both c-IAP1 and c-IAP2. The immunogen is located within amino acids 540 - 590 of cIAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| cIAP antibody can be used for the detection of c-IAP by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BIRC2 |
|---|---|
| Alternative Names | cIAP Antibody: API1 MIHB HIAP2 RNF48 cIAP1 Hiap-2 c-IAP1 API1 IAP2 Baculoviral IAP repeat-containing protein 2 C-IAP1 IAP-2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (93%) |
Application
Immunohistochemistry of cIAP in human lung cells with cIAP antibody at 10 μg/mL.
Application
Immunofluorescence of cIAP in Human Lung cells with cIAP antibody at 20 μg/mL.
Application
Western blot analysis of c-IAP in human lung lysate with c-IAP antibody at 1 (lane A), 2 (lane B), and 4 (lane C) μg/mL, respectively.| Positive Control | 1) Cat. No. 1331 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | cIAP antibody can be used for the detection of c-IAP by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | cIAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | cIAP Antibody is supplied in PBS containing 0.02% sodium azide. |
