WB
Western blot analysis of GATM using anti-GATM antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GATM antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of GATM using anti-GATM antibody . GATM was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GATM Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of GATM using anti-GATM antibody. GATM was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-GATM Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of GATM using anti-GATM antibody. GATM was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GATM Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of THP-1 cells using anti-GATM antibody. Overlay histogram showing THP-1 cells stained with Rabbit polyclonal antibody to GATM (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GATM Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to GATM |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GATM recombinant protein (Position: M1-E279). Human GATM shares 95.3% and 95% amino acid (aa) sequence identity with mouse and rat GATM, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GATM |
|---|---|
| Gene Synonyms | AT RFS AGAT FRTS CCDS3 FRTS1 |
| Gene Full Name | glycine amidinotransferase |
| Gene Summary | This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by cognitive disability, language impairment, and behavioral disorders. [provided by RefSeq, Jul 2008] |
| Alternative Names | AGAT AT GATM Transamidinase |
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Cytoplasm,Membrane,Mitochondrion,Mitochondrion inner membrane. |
WB
Western blot analysis of GATM using anti-GATM antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GATM antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of GATM using anti-GATM antibody . GATM was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GATM Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of GATM using anti-GATM antibody. GATM was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-GATM Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of GATM using anti-GATM antibody. GATM was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GATM Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of THP-1 cells using anti-GATM antibody. Overlay histogram showing THP-1 cells stained with Rabbit polyclonal antibody to GATM (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GATM Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
