WB
Western blot analysis of various lysates, using MMRN1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC):U-251 MG Exposure time: 180s.ICC/IF
Immunofluorescence analysis of U2OS cells using MMRN1 Rabbit pAb. Secondary antibody: 488-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.| Product Name | MMRN1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MMRN1 |
|---|---|
| Gene Synonyms | ECM MMRN GPIa* EMILIN4 |
| Gene Full Name | multimerin 1 |
| Gene Summary | Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. It is comprised of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. It may also have functions as an extracellular matrix or adhesive protein. Recently, patients with an unusual autosomal-dominant bleeding disorder (factor V Quebec) were found to have a deficiency of platelet multimerin. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 138kDa(Observed MW 180kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of various lysates, using MMRN1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC):U-251 MG Exposure time: 180s.
ICC/IF
Immunofluorescence analysis of U2OS cells using MMRN1 Rabbit pAb. Secondary antibody: 488-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
