IF-F
Application: IHC-Fr, Species: Mouse, Site: Cerebellum, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Not required,WB
Western blot analysis of HOPX / HOD on different lysates with Rabbit anti-HOPX / HOD antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-HOPX / HOD antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | HOPX / HOD Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human aa 1-73. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:2000 IF-F:1:500 IHC:1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | Hopx |
|---|---|
| Gene Synonyms | Hod Hop Ob1 Obl Hdop Toto Cameo 1110018K11Rik 1200015P04Rik 2300002F06Rik |
| Gene Full Name | HOP homeobox |
| Gene Summary | Enables histone deacetylase regulator activity. Involved in positive regulation of gap junction assembly. Acts upstream of or within several processes, including lung alveolus development; positive regulation of skeletal muscle tissue regeneration; and trophectodermal cell differentiation. Located in nucleus. Is expressed in several structures, including central nervous system; epithelium; heart; trophectoderm; and urinary system. Orthologous to human HOPX (HOP homeobox). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CAMEO antibody heart odd homeobox 1 protein antibody HOD antibody Homeodomain-only protein antibody HOP antibody HOP homeobox antibody HOP_HUMAN antibody HOPX antibody LAGY antibody Lung cancer-associated Y protein antibody NECC1 antibody Not expressed in choriocarcinoma clone 1 antibody Not expressed in choriocarcinoma protein 1 antibody OB1 antibody odd homeobox 1 protein antibody Odd homeobox protein 1 antibody OTTHUMP00000158970 antibody SMAP31 antibody TOTO antibody |
| Molecular Weight(MW) | 8 kDa(Observed band size: 14 kDa) |
| Cellular Localization | Nucleus, Cytoplasm. |
IF-F
Application: IHC-Fr, Species: Mouse, Site: Cerebellum, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Not required,
WB
Western blot analysis of HOPX / HOD on different lysates with Rabbit anti-HOPX / HOD antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-HOPX / HOD antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IF-F:1:500 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
