WB
Western blot analysis of lysates from Rat heart, using HOPX Rabbit pAb at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.IF-P
Immunofluorescence analysis of paraffin embedded Human skin using HOPX Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Product Name | HOPX Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PWB |
| WB:1:500-1:1000 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HOPX |
|---|---|
| Gene Synonyms | HOD HOP OB1 LAGY TOTO CAMEO NECC1 SMAP31 |
| Gene Full Name | HOP homeobox |
| Gene Summary | The protein encoded by this gene is a homeodomain protein that lacks certain conserved residues required for DNA binding. It was reported that choriocarcinoma cell lines and tissues failed to express this gene, which suggested the possible involvement of this gene in malignant conversion of placental trophoblasts. Studies in mice suggest that this protein may interact with serum response factor (SRF) and modulate SRF-dependent cardiac-specific gene expression and cardiac development. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Feb 2009] |
| Alternative Names | HOD HOP OB1 LAGY TOTO CAMEO NECC1 SMAP31 HOPX |
| Molecular Weight(MW) | 14kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of lysates from Rat heart, using HOPX Rabbit pAb at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
IF-P
Immunofluorescence analysis of paraffin embedded Human skin using HOPX Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Application Notes | WB:1:500-1:1000 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
