raised against
AcNPV extracellular nonoccluded virus (NOV)
Key Feature
Clonality
Monoclonal
Isotype
IgG2b,k
Host Species
Mouse
Tested Applications
FCIPWB
WB:1:100-1:1000 IP:1-2 µg per 100-500 µg of
total protein (1 ml of cell lysate) FC:1 µg per 1 x 106 cells
Species Reactivity
Virus
Concentration
0.2mg/ml
Purification
Affinity purified
Target Information
Target
baculovirus envelope protein gp64
Molecular Weight(MW)
64-67 kDa
Function
Viruses are obligate intracellular parasites which, lacking the cellular
machinery for self-reproduction, can reproduce only by invading and controlling
other cells. Virus particles are composed of a nucleic acid genome consisting
of either DNA or RNA encapsidated with a protein coat. The Baculovirus
envelope protein gp64 is a phosphoglycoprotein located on the surface of
infected cells and budded virions. This protein may be involved in fusion of the
viral envelope with the endosomal membrane. It also contains N-linked glyco
sylation sites and hydrophobic C- and N-termini, a characteristic of signal and
membrane anchor motifts in envelope glycoproteins.
Application
WB
Western blot analysis of recombinant gp64 Autographa californica nucleopolyhedrovirus.
Application Notes
WB:1:100-1:1000 IP:1-2 µg per 100-500 µg of
total protein (1 ml of cell lysate) FC:1 µg per 1 x 106 cells
Additional Information
Form
Liquid
Storage Instructions
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.
Storage Buffer
Each vial contains 200 µg IgG2b kappa light chain in 1.0 ml of PBS with < 0.1%
sodium azide and 0.1% gelatin.