WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPRD1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPRD1B.IHC
Immunohistochemical staining of paraffin embedded Human colon tissue within the normal limits using anti-RPRD1B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Product Name | C20orf77 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human RPRD1B(NP_067038) produced in HEK293T cell. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:4000 IHC:1:150 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | RPRD1B |
|---|---|
| Gene Synonyms | K-H CREPT NET60 C20orf77 Kub5-Hera dJ1057B20.2 |
| Gene Full Name | regulation of nuclear pre-mRNA domain containing 1B |
| Gene Summary | Enables RNA polymerase II complex binding activity and identical protein binding activity. Involved in several processes, including RNA polymerase II promoter clearance; positive regulation of cell population proliferation; and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of transcription preinitiation complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | C20orf77 CREPT dJ1057B20.2 NET60 |
| Molecular Weight(MW) | 36.7 kDa |
| Cellular Localization | Nucleus. |
WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPRD1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPRD1B.
IHC
Immunohistochemical staining of paraffin embedded Human colon tissue within the normal limits using anti-RPRD1B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Application Notes | WB:1:4000 IHC:1:150 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
