WB
Western blot analysis of extracts of various lysates, using ATAD3A/ATAD3B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer:3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.ICC/IF
Immunofluorescence analysis of U20S cells using ATAD3A/ATAD3B antibody at dilution of 1:100 .Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of L929 cells using ATAD3A/ATAD3B antibody at dilution of 1:100 .Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of H9C2 cells using ATAD3A/ATAD3B antibody at dilution of 1:100 .Blue: DAPI for nuclear staining.| Product Name | ATAD3A/ATAD3B polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human ATAD3B(NP_114127.3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATAD3B |
|---|---|
| Gene Synonyms | TOB3 AAA-TOB3 |
| Gene Full Name | ATPase family AAA domain containing 3B |
| Gene Summary | The protein encoded by this gene is localized to the mitochondrial inner membrane, where it can bind to a highly-related protein, ATAD3A. ATAD3A appears to interact with matrix nucleoid complexes, and the encoded protein negatively regulates that interaction. This gene is expressed almost exclusively in pluripotent embryonic stem cells and some cancer cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015] |
| Alternative Names | ATAD3B AAA-TOB3 TOB3 ATPase family |
| Molecular Weight(MW) | 68 KDa |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion inner membrane. |
WB
Western blot analysis of extracts of various lysates, using ATAD3A/ATAD3B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer:3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of U20S cells using ATAD3A/ATAD3B antibody at dilution of 1:100 .Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of L929 cells using ATAD3A/ATAD3B antibody at dilution of 1:100 .Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of H9C2 cells using ATAD3A/ATAD3B antibody at dilution of 1:100 .Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
