WB
Western blot analysis of lysates from PC12 cells(heat-shock,45min treatment), using MBNL2 Antibody at 1/1000 dilution. 5ug/NC membrane strip. Exposure for 30s with ECL Kit. Bands result from membrane strip incubation.IHC
Rabbit polyclonal antibody to MBNL2 at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.| Product Name | Rabbit polyclonal antibody to MBNL2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human MBNL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MBNL2 |
|---|---|
| Gene Synonyms | MBLL MBLL39 PRO2032 |
| Gene Full Name | muscleblind like splicing regulator 2 |
| Gene Summary | This gene is a member of the muscleblind protein family which was initially described in Drosophila melanogaster. This gene encodes a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. [provided by RefSeq, Mar 2012] |
| Alternative Names | DKFZp781H1296 MBLL MBLL39 Mbnl2 MBNL2_HUMAN MGC120625 MGC120626 MGC120628 Muscleblind like 2 muscleblind like protein 1 muscleblind like protein 2 muscleblind like protein like 39 muscleblind like splicing regulator 2 Muscleblind-like protein 1 Muscleblind-like protein 2 Muscleblind-like protein-like 39 Muscleblind-like protein-like OTTHUMP00000178763 PRO2032 RP11-128N14.1 |
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
WB
Western blot analysis of lysates from PC12 cells(heat-shock,45min treatment), using MBNL2 Antibody at 1/1000 dilution. 5ug/NC membrane strip. Exposure for 30s with ECL Kit. Bands result from membrane strip incubation.
IHC
Rabbit polyclonal antibody to MBNL2 at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
