WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GAD-65/67 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Rat brain was stained with anti-GAD-65/67 Rabbit antibody.IHC
Human pancreas was stained with anti-GAD-65/67 Rabbit antibody.IF-P
Immunofluorescence analysis of human testis. 1,primary antibody(green) was diluted at 1:200(4℃,overnight). 2, AFlour 488 labled Secondary antibody was diluted at 1:300(room temperature, 50min).| Product Name | GAD-65/67 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:10000-1:50000 IHC:1:200-1:1000 IF-P:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GAD1 |
|---|---|
| Gene Synonyms | GAD SCP CPSQ1 DEE89 GAD-67 |
| Gene Full Name | glutamate decarboxylase 1 |
| Gene Summary | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008] |
| Alternative Names | GAD1 GAD GAD67 Glutamate decarboxylase 1 67 kDa glutamic acid decarboxylase GAD-67 Glutamate decarboxylase 67 kDa isoform GAD2 GAD65 Glutamate decarboxylase 2 65 kDa glutamic acid decarboxylase GAD-65 Glutamate decarboxylase 65 |
| Molecular Weight(MW) | 65,67kDa |
| Cellular Localization | intracellular ,plasma membrane ,vesicle membrane ,presynaptic active zone ,clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GAD-65/67 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Rat brain was stained with anti-GAD-65/67 Rabbit antibody.
IHC
Human pancreas was stained with anti-GAD-65/67 Rabbit antibody.
IF-P
Immunofluorescence analysis of human testis. 1,primary antibody(green) was diluted at 1:200(4℃,overnight). 2, AFlour 488 labled Secondary antibody was diluted at 1:300(room temperature, 50min).| Application Notes | WB:1:10000-1:50000 IHC:1:200-1:1000 IF-P:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
