WB
Western blot analysis of NFU1 using anti-NFU1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFU1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of NFU1 using anti-NFU1 antibody and anti-Beta Tubulin antibody. NFU1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NFU1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of A549 cells using anti-NFU1 antibody. Overlay histogram showing A549 cells stained with Rabbit polyclonal antibody to NFU1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFU1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to NFU1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human NFU1 recombinant protein (Position: Q42-P254). Human NFU1 shares 93% amino acid (aa) sequence identity with mouse NFU1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFU1 |
|---|---|
| Gene Synonyms | Nfu NifU HIRIP MMDFS MMDS1 NIFUC SPG93 CGI-33 HIRIP5 |
| Gene Full Name | NFU1 iron-sulfur cluster scaffold |
| Gene Summary | This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
| Alternative Names | NFU1 HIRIP5 CGI-33 NFU1 iron-sulfur cluster scaffold homolog mitochondrial HIRA-interacting protein 5 |
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cytoplasm,Mitochondrion. |
WB
Western blot analysis of NFU1 using anti-NFU1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFU1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of NFU1 using anti-NFU1 antibody and anti-Beta Tubulin antibody. NFU1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NFU1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of A549 cells using anti-NFU1 antibody. Overlay histogram showing A549 cells stained with Rabbit polyclonal antibody to NFU1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFU1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
