WB
Western blot analysis of various lysates using DLL3 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): BxPC-3. Exposure time: 10 s.IHC
Immunohistochemistry analysis of paraffin embedded Human malignant melanoma tissue using DLL3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Confocal imaging of TT cells and BxPC-3 (negative) cells using DLL3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488 conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.IP
Immunoprecipitation of DLL3 from 300 µg extracts of TT cells was performed using 0.15 µg of DLL3 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using DLL3 Rabbit mAb at a dilution of 1:5000.| Product Name | DLL3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000 - 1:10000 IHC:1:200 - 1:800 ICC/IF:1:100 - 1:400 IP: 0.15 μg - 0.3 μg antibody for 200 μg - 400 μg extracts of whole cells |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DLL3 |
|---|---|
| Gene Synonyms | SCDO1 |
| Gene Full Name | delta like canonical Notch ligand 3 |
| Gene Summary | This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | SCDO1 |
| Molecular Weight(MW) | 61 kDa/65 kDa(Observed band size:65 kDa) |
| Cellular Localization | Membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates using DLL3 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): BxPC-3. Exposure time: 10 s.
IHC
Immunohistochemistry analysis of paraffin embedded Human malignant melanoma tissue using DLL3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Confocal imaging of TT cells and BxPC-3 (negative) cells using DLL3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488 conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
IP
Immunoprecipitation of DLL3 from 300 µg extracts of TT cells was performed using 0.15 µg of DLL3 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using DLL3 Rabbit mAb at a dilution of 1:5000.| Application Notes | WB:1:2000 - 1:10000 IHC:1:200 - 1:800 ICC/IF:1:100 - 1:400 IP: 0.15 μg - 0.3 μg antibody for 200 μg - 400 μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
