WB
Western blot analysis using DLL3 mouse mAb against Hela (1)cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using DLL3 mouse mAb with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using DLL3 mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using DLL3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher.FC
Flow cytometric analysis of C6 cells using DLL3 mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to DLL3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human DLL3 (AA: EXTRA(27-226)) expressed in E. Coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 FC:1:200-1:400 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DLL3 |
|---|---|
| Gene Synonyms | SCDO1 |
| Gene Full Name | delta like canonical Notch ligand 3 |
| Gene Summary | This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | SCDO1 |
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Membrane. |
WB
Western blot analysis using DLL3 mouse mAb against Hela (1)cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using DLL3 mouse mAb with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using DLL3 mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using DLL3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher.
FC
Flow cytometric analysis of C6 cells using DLL3 mouse mAb (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 FC:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
