WB
Western blot analysis of various lysates using Heparanase 1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.ICC/IF
Immunofluorescence analysis of HeLa cells using Heparanase 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of NIH/3T3 cells using Heparanase 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Heparanase 1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500 - 1:2000 ICC/IF:1:50 - 1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HPSE |
|---|---|
| Gene Synonyms | HPA HPA1 HPR1 HSE1 HPSE1 |
| Gene Full Name | heparanase |
| Gene Summary | Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Alternative Names | HPA HPA1 HPR1 HSE1 HPSE1 Heparanase 1 |
| Molecular Weight(MW) | 61 kDa(Observed band size:50 kDa/65 kDa) |
| Cellular Localization | Lysosome membrane, Nucleus, Peripheral membrane protein, Secreted. |
WB
Western blot analysis of various lysates using Heparanase 1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
ICC/IF
Immunofluorescence analysis of HeLa cells using Heparanase 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of NIH/3T3 cells using Heparanase 1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500 - 1:2000 ICC/IF:1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
