WB
Positive control:HepG2, Hela, SVT2, COS7, MDCK, MCF-7 cell lysates. Recommended dilution: 200-1000. Gel: 8%SDS-PAGE . Lysate: 35μg . Lane 1-9: HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates . Primary antibody: PCDH7 Antibody at dilution 1/200 . Secondary antibody: Goat anti mouse IgG at 1/8000 dilution . Exposure time: 15 minutes.IHC
Predicted cell location: Cell membrane, Membrane . Positive control: human endometrium tissue . Recommended dilution: 30-150. The image is immunohistochemistry of paraffin-embedded human endometrium tissue using PCDH7 Antibody at dilution 1/40. (Original magnification: ×200).ICC/IF
Predicted cell location: Cell membrane, Membrane . Positive control: COS7 cells transiently transfected by pCMV6-ENTRY . Recommended dilution: 100-200 . The image is immunofluorescence of COS7 cells transiently transfected by pCMV6-ENTRY using PCDH7 Antibody at dilution 1/100.| Product Name | Anti-PCDH7 mouse monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Fusion protein of human PCDH7. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:200-1:1000 IHC:1:30-1:150 ICC/IF:1:100-1:200 |
|
| Species Reactivity | DogHumanMonkeyMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCDH7 |
|---|---|
| Gene Synonyms | BHPCDH BH-Pcdh PPP1R120 |
| Gene Full Name | protocadherin 7 |
| Gene Summary | This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a protein with an extracellular domain containing 7 cadherin repeats. The gene product is an integral membrane protein that is thought to function in cell-cell recognition and adhesion. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq, Jul 2008] |
| Alternative Names | BH-Pcdh BHPCDH PPP1R120 |
| Molecular Weight(MW) | 116 kDa |
| Cellular Localization | Cell membrane,Membrane. |
WB
Positive control:HepG2, Hela, SVT2, COS7, MDCK, MCF-7 cell lysates. Recommended dilution: 200-1000. Gel: 8%SDS-PAGE . Lysate: 35μg . Lane 1-9: HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates . Primary antibody: PCDH7 Antibody at dilution 1/200 . Secondary antibody: Goat anti mouse IgG at 1/8000 dilution . Exposure time: 15 minutes.
IHC
Predicted cell location: Cell membrane, Membrane . Positive control: human endometrium tissue . Recommended dilution: 30-150. The image is immunohistochemistry of paraffin-embedded human endometrium tissue using PCDH7 Antibody at dilution 1/40. (Original magnification: ×200).
ICC/IF
Predicted cell location: Cell membrane, Membrane . Positive control: COS7 cells transiently transfected by pCMV6-ENTRY . Recommended dilution: 100-200 . The image is immunofluorescence of COS7 cells transiently transfected by pCMV6-ENTRY using PCDH7 Antibody at dilution 1/100.| Application Notes | WB:1:200-1:1000 IHC:1:30-1:150 ICC/IF:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20℃. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
