WB
WB analysis using Vinculin Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with Vinculin antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human heart using Vinculin Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human heart tissue slide using Vinculin antibody at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
IHC staining of rat heart using Vinculin Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using Vinculin antibody at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
IF Staining of hTERT-RPE1 using Vinculin Monoclonal antibody. Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Vinculin antibody at dilution of 1:2000 and Multi-rAb Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L).| Product Name | Vinculin Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human Vinculin protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:50000 IHC:1:1000-1:4000 ICC/IF:1:1000-1:4000 |
|
| Species Reactivity | HumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | VCL |
|---|---|
| Gene Synonyms | MV MVCL VINC CMD1W CMH15 HEL114 |
| Gene Full Name | vinculin |
| Gene Summary | Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | 3A11F8 CMD1W Metavinculin MV MVCL |
| Molecular Weight(MW) | 1133 aa, 124 kDa |
| Cellular Localization | Cell junction,Cell membrane,Cell projection,Cytoplasm,Cytoskeleton,Membrane. |
WB
WB analysis using Vinculin Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with Vinculin antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human heart using Vinculin Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human heart tissue slide using Vinculin antibody at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
IHC staining of rat heart using Vinculin Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using Vinculin antibody at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
IF Staining of hTERT-RPE1 using Vinculin Monoclonal antibody. Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Vinculin antibody at dilution of 1:2000 and Multi-rAb Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L).| Application Notes | WB:1:5000-1:50000 IHC:1:1000-1:4000 ICC/IF:1:1000-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
