WB
Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-PMEL antibody. The HRP-conjugated Goat anti-Mouse IgG (H + L) antibody was used to detect the antibody.IHC
Human colon was stained with anti-Vinculin Mouse antibody.| Product Name | Vinculin Mouse mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from human Vinculin. AA range: 100-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | VCL |
|---|---|
| Gene Synonyms | MV MVCL VINC CMD1W CMH15 HEL114 |
| Gene Full Name | vinculin |
| Gene Summary | Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | VCL Vinculin Metavinculin |
| Molecular Weight(MW) | 124 kDa |
| Cellular Localization | Membranous , Cytoplasmic. |
WB
Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-PMEL antibody. The HRP-conjugated Goat anti-Mouse IgG (H + L) antibody was used to detect the antibody.
IHC
Human colon was stained with anti-Vinculin Mouse antibody.| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
