WB
WB analysis using CA12 Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CA12 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.ICC/IF
IF Staining of A549 using CA12 Monoclonal antibody. Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CA12 antibody at dilution of 1:2000 and 488-Conjugated Goat Anti-Mouse IgG(H+L).| Product Name | CA12 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CA12 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:5000-1:50000 ICC/IF:1:1000-1:4000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CA12 |
|---|---|
| Gene Synonyms | CAXII CA-XII T18816 HsT18816 |
| Gene Full Name | carbonic anhydrase 12 |
| Gene Summary | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014] |
| Alternative Names | CA XII CA12 Carbonate dehydratase XII Carbonic anhydrase 12 carbonic anhydrase XII CAXII FLJ20151 HsT18816 Tumor antigen HOM RCC 3.1.3 |
| Molecular Weight(MW) | 39 kDa(Observed band size:38-45 kDa) |
| Cellular Localization | Cell membrane,Membrane. |
WB
WB analysis using CA12 Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CA12 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
ICC/IF
IF Staining of A549 using CA12 Monoclonal antibody. Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CA12 antibody at dilution of 1:2000 and 488-Conjugated Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:5000-1:50000 ICC/IF:1:1000-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
