WB
Western blot analysis of lysates from Hela cells(starvation 24h treatment), using DCN Antibody at 1/1000 dilution. 5ug/NC membrane strip. Exposure for 2s with ECL Kit. Bands result from membrane strip incubation.IHC
Rabbit polyclonal antibody to DCN at 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.IHC
Rabbit polyclonal antibody to DCN at 1/100 staining Mouse ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to DCN |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human DCN, corresponding to a region within the internal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCN |
|---|---|
| Gene Synonyms | CSCD PG40 PGII PGS2 DSPG2 SLRR1B |
| Gene Full Name | decorin |
| Gene Summary | This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients. [provided by RefSeq, Nov 2015] |
| Alternative Names | Bone proteoglycan II CSCD DCN DCN protein Decorin Decorin proteoglycan Dermatan sulphate proteoglycans II DKFZp686J19238 DSPG 2 DSPG2 PG 40 PG II PG S2 PG-S2 PG40 PGII PGS 2 PGS2 PGS2_HUMAN Proteoglycan core protein SLRR1B Small leucine rich protein 1B |
| Molecular Weight(MW) | 40 kDa(Observed band size: 40,70-100kDa) |
| Cellular Localization | Secreted>Extracellular space>Extracellular matrix. |
WB
Western blot analysis of lysates from Hela cells(starvation 24h treatment), using DCN Antibody at 1/1000 dilution. 5ug/NC membrane strip. Exposure for 2s with ECL Kit. Bands result from membrane strip incubation.
IHC
Rabbit polyclonal antibody to DCN at 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
IHC
Rabbit polyclonal antibody to DCN at 1/100 staining Mouse ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
